Journal: Nature communications

Article Title: A truncated variant of the ribosome-associated trigger factor specifically contributes to plant chloroplast ribosome biogenesis.

doi: 10.1038/s41467-025-55813-1

Figure Lengend Snippet: Fig. 6 | Ribosome biogenesis defects caused by Tig2 deletion. a Left: Immuno- blot monitoring accumulation of selected chloroplast proteins in Col-0 and trigger factor mutant lines. Protein samples were collected from young leaves after 21 days of cold-exposure; right panel: quantification of bands. Mean values derive from three biological replicates, error bars denote SD, two-sided Student’s t-test p-values are given, “n.s.” not significant. b Seedlings were grown for 10 days (DAG) on plates

Article Snippet: For labeling, 1 μg of the Tig2 antibody and IgG Rabbit antibody (Thermo Fisher, Germany), respectively, were labeledusing the FlexibleCoralite 488AntibodyLabelingKit according to themanufacturer’s instructions (Proteintech, Germany) and used at the 1:250 dilution in the hybridization buffer54.

Techniques: Mutagenesis